Indole-3-Carbinol Inhibits Laryngeal Cancer Growth Through Cell Cycle Arrest

Chenggang Mao

doi:10.11648/j.jdmp.20190502.11

Abstract

The growth of a variety of tumors are inhibited by indole-3-carbinol (I3C) obviously. But, its role in laryngeal cancer is not clear. The goal of this study was to research the probable roles that laryngeal cancer cell apoptosis and proliferation Hep-2 was influenced by I3C. I3C dose-dependently therapy obviously inhibited Hep-2cell proliferation, and, I3C promoted apoptosis and induced cell morphological changes at 100, 200, 300, 400 μM doses. We discovered that I3C shows anticancer effect through various signal pathways after Hep-2 cells I3C therapy. In Hep-2laryngeal cancer cell line, through decreasing cell cycle-related proteins that include cyclin D1, CDK6, CDK4, and pRb, G1 arrest was induced by I3C. Apart from this, BALB/c nude mice constructed tumor-bearing mouse models. BALB/c nude mice were divided into three groups: treated with I3C, untreated control group and pretreated with I3C. After 8 weeks treatment, the untreated control group developed bigger tumors compared to mice treated or pretreated with I3C, and in the tumors such as cyclin D1, CDK6, CDK4 and pRb cell cycle-related proteins were obviously decreased. Further, the study result showed there was no harmful side effect in the heart, liver and kidney of the I3C-treated nude mice. In conclusion, both in vivo and in vitro I3C inhibited proliferation and induced the Hep-2 cells apoptosis, and showed low toxicity to normal cells. By suppressing the expression of cyclin families and CDK, we deduce that I3C can inhibit the Hep-2 cells growth in vitro. On normal organs and tissues, the I3C had no toxic effects and was safe.

Highlights

In the head and neck, in highly developed countries, laryngeal cancer which is one of the leading malignant tumors is reported to have a higher incidence [1]
In some signal transduction pathways that were associated with the cell growth inhibition, the anticancer activity of I3C is reflected. the inhibition effects of I3C have been evaluated by previous studies on the PI3K/Akt pathway [2, 7, 8] and nuclear factor-kB [9] signal transduction pathways
The Growth of Laryngeal Cancer Cells Were Inhibited by I3C

Summary

Introduction

In the head and neck, in highly developed countries, laryngeal cancer which is one of the leading malignant tumors is reported to have a higher incidence [1]. There are many choices including the traditional ways of chemotherapy, radiotherapy and surgery that can be used to treat laryngeal cancer. A less toxic and highly effective way is lacking, for the cancer treatment, many chemotherapeutic drugs are available. The beneficial effects of intake of fruits and vegetables have been shown in lowering the incidence of cancers in recent dietary and epidemiological studies. In some signal transduction pathways that were associated with the cell growth inhibition, the anticancer activity of I3C is reflected. The inhibition effects of I3C have been evaluated by previous studies on the PI3K/Akt pathway [2, 7, 8] and nuclear factor-kB [9] signal transduction pathways. Mitogen-activated protein kinases (MAPK) expression was been down-regulated by I3C

Objectives

Methods

Results

Conclusion