Abstract
Indocyanine green (ICG) is an infrared fluorescent dye with widespread use in clinical applications, including hepatic function tests, cardiac output monitoring, and ophthalmic angiography. Using Xenopus laevis oocytes under voltage clamp, we have found that ICG is voltage-sensitive and capable of responding to changes in cellular membrane potential with a roughly linear voltage dependency. ICG's voltage response does not display the sub-millisecond kinetics characteristic of electrochromic dyes; however, it is faster than many so-called slow-response voltage probes. Tests of oocytes performed under current clamp-like conditions show that ICG can clearly follow action potentials produced at approximately 70 Hz. ICG has low expected cytotoxicity; an infrared wavelength amenable to deep tissue imaging; and the ability to label excitable cells, including cardiac myocytes and neurons. Consequently, the voltage-sensitivity of ICG presented here may have significant implications for novel applications in research and the clinic. Support: NIH GM030376.
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
