Abstract
Individual semen samples from 29 bulls in routine artificial breeding service were tested for their capability to achieve in vitro fertilization. Ejaculated semen was diluted, .1ml of semen in 2ml of a modified Tyrode's medium with an osmolality of 340 mOsmol/kg, washed thrice, incubated 3h at 37°C before being used for in vitro fertilization, or incubated 4h and 8h before assessment of motility, capacitation, and acrosome integrity. The degree of variability in percentage of oocytes fertilized was assessed along with several factors that might contribute to this variation. Variation among bulls was not significantly different. Variation from one replicate to another was high. Variation was found in motility, capacitation, and frequency of acrosome reaction, but these variables were not significantly correlated to fertilization rate in vitro.
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