Individual Typological Features of Postresuscitative Cerebral Changes: Role of Heat Shock Proteins HSP70

Abstract

Objective: to establish how the protective properties of HSP70 family proteins are realized in the postresuscitative period depending on the organism’s individual typological features. Materials and methods. Neuronal populations of the CA1 and CA4 region of the hippocampus, cerebral sensorimotor cortex V and Purkinje’s cells of the cerebellum were comparatively morpho-metrically and immunohistochemically studied in the rats who had sustained 12-minute clinical death and differed in the results of active avoidance conditioning (AAC). Immunological responsivness to heat-shock proteins was determined by peroxidase-antiperoxidase assay using polyclonal antibodies to HSP70. The density and composition of the neuronal populations were ascertained by morphometric analysis. Results. The control animals having varying capacity for AAC were found to have differences in the composition of neuronal populations of hippocampal region CA4 and cerebellar Purkinje’s cells. Marked postresuscitative neuronal damages were only detected in the high-learned rats. In the controls, immunological responsivness to HSP70 in the CA1 and CA4 pyramidal cell populations of the hippocampus was found to be greater in the high-learned rats than that in the low-learned ones. There was only a postresuscitative increase in immunological responsivness to HSP70 in the CA1 and CA4 pyramidal cell populations of the hippocampus in the low-learned rats. Conclusion. By and large, the results of the present study suggest that the immunological responsivness of neuronal populations to HSP70 is linked to the individual typological features of the organism. There are differences in the immunological responsivness of neuronal populations to HSP70 between the animals having varying ability to learn in the controls and in the postresuscitative period. The specific features of exhibition of the neuroprotective properties of HSP70 depending on individual typological features of the organism are one of the important factors of different cerebral resistance to postresuscitative damages. Key words: cardiac arrest, individual typological features, HSP70, immunohistochemistry, neuronal populations.