Abstract
The effect of a microRNA on the levels of its target mRNAs can be measured within a single gene expression profile
Highlights
MicroRNAs are oligoribonucleotides with an important role in regulation of gene expression at the level of translation
Detection of miRNA signatures within endogenous gene expression profiles miRNAs can down-regulate target mRNAs; one would expect that the target genes of a highly expressed miRNA might be expressed at a significantly lower level than those of a lowly expressed miRNA
We tested the ability of three analytical approaches to detect the effects of variable endogenous miRNA expression on mRNA levels
Summary
MicroRNAs (miRNAs) are oligoribonucleotides with an important role in regulation of gene expression at the level of translation. The validity of miRNA target gene predictions is difficult to assess at the protein level. To determine whether a general lowering of predicted target gene mRNA expression by endogenous miRNAs was detectable within microarray gene expression profiles. Target genes are determined by sequence complementarity between the 3' untranslated region (UTR) and the mature miRNA, in a 6 bp 'seed' region [1,2]. 'TargetScan' [4,5] searches for conserved 8-mer and 7-mer sites in 3' UTRs that match the seed region of a known miRNA. It is possible, to obtain lists of the potential target mRNAs for each miRNA. Plant miRNAs, which are often perfectly matched to their target
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