Abstract
Individual cell growth rates enhance our understanding of microbial roles in regulat- ing organic matter flux in marine and other aquatic systems. We devised a protocol to microscop- ically detect and quantify bacteria undergoing replication in seawater using the thymidine analog 5-ethynyl-2'-deoxyuridine (EdU), which becomes incorporated into bacterial DNA and is detected with a 'click' chemistry reaction in 3 orders of magnitude, wherein the most intensely labeled cells comprised most of a sam- ple's sum community EdU signal, e.g. 26% of cells comprised 80% of the sum signal. This ability to rapidly detect and quantify signals in labeled DNA is an important step toward a robust approach for the determination of single-cell growth rates in natural assemblages and for linking growth rates with microscale biogeochemical dynamics.
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