Indium-111-Oxine???Labeled Platelet Scintigraphic Images in the Assessment of Thrombogenicity in Small-Caliber Prosthetic Vascular Grafts

Abstract

Anti-thrombogenicity is one of the essential factors for successful development of small-caliber prosthetic vascular grafts, and its accurate evaluation is important. The purpose of this study was to investigate the efficacy of indium-111-oxine-labeled (In) platelet scintigraphic images in the assessment of graft thrombogenicity. Fibrin-coated knitted polyester vascular prostheses, 2 mm in diameter and 2 cm in length, were implanted in the bilateral common carotid arteries of 18 Japanese white rabbits. The grafts were explanted on postoperative days 1, 3, 7, 10, 14, and 30 (six grafts on each of the days), and In platelet scintigraphy was performed with visual, semiquantitative, and quantitative analysis. The relationships among the three analyses were evaluated. Platelet depositions on the luminal surface were clearly visualized with In-platelet scintigraphy if the number of In platelets was more than 4.8 x 10 counts per minute, even if a macroscopic thrombus formation was not observed. There was a highly significant correlation between the graft region of interest on the images and the total platelet deposition (p<.0001). The images in the thrombotic grafts have shown a very strong In-platelet accumulation, but there were some limitations in the In-platelet scintigraphic images. In-platelet scintigraphic images are useful for evaluating platelet deposition in small-caliber vascular prostheses if they are performed with a proper understanding of their limitations.