Indirect Spectrophotometric Determination of Famotidine and Ciprofloxacin Hydrochloride in Pharmaceuticals Using N-Bromosuccinimde and Janus Green B Dye.

Abstract

An indirect simple and sensitive spectrophotometric method has been developed for the determination of famotidine (FAM) and ciprofloxacin hydrochloride (CIP) in pure and pharmaceutical dosages. The method is based on the oxidation of FAM and CIP with known excess of N-Bromosuccinimde in acidic medium and subsequent occupation of unreacted oxidant in decolorization of Janus green B dye and measure the absorbance of residual dye at 618 nm. Calibration curves of residual Janus green B dye in the presence of FAM or CIP were rectilinear over the ranges 1.0-10.0 and 1.0-7.5 µg/ml with molar absorptivity 3.61×104 and 5.14×104 l.mol-1.cm-1 respectively. The accuracy (average recovery) was ranged between 99.68 and 100.23% and precision (RSD%) is less than 3.80%. The developed method was successfully applied for the determination of the studied drugs in their pharmaceutical preparations. The results obtained are in agreement with certified values of pharmaceutical preparations and also with standard addition procedure and standard method applied in state company for drugs industry and medical appliance,SDI.