Abstract
A new immunoassay method using surface plasmon resonance (SPR) coupled with online, in-tube, solid-phase microextraction (SPME) was developed and applied to detect estradiol in human serum and seawater using an indirect inhibitive immunoassay format. The binding of antibody was inhibited by estradiol in a dose-dependent manner, and the calibration curve was generated by linear fit over the range of 0.3125–20.0 ng/ml, with a correlation coefficient of R2 = 0.99996. The detection limit (S/N = 3) was 0.17 ng/ml. This study demonstrates, for the first time, the feasibility of in-tube SPME-SPR to determine estradiol in human serum and seawater.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
