Abstract
Monoclonal antibodies were used to detect influenza A and B in clinical specimens by an indirect immunofluorescence assay using Madden-Darby canine kidney monolayers in shell vials. Twenty-nine influenza virus isolates, including 23 type A and six type B, were detected in conventional tube cell culture at an average time of 4.2 days. Of these isolates, 18 (62%) were detected by indirect immunofluorescence 24 hours after inoculation, and 24 isolates (83%) were detected at 48 hours. Of 30 culture-negative specimens tested, influenza A was detected in one specimen by immunofluorescence. The shell vial assay is useful for rapid detection of influenza types A and B in clinical specimens, and the sensitivity is increased by staining at 48 hours in addition to 24 hours.
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