Abstract
Indirect double sandwich ELISA was examined for use in assay systems to detect enterotoxins A,B,C 1 and D in foods implicated in food poisoning outbreaks. With this simple, sensitive and rapid test, it was possible to detect as low as 1 ng of enterotoxins per g of food sample. This system uses a monoclonal antibody to coat polystyrene plates and polyclonal rabbit antibodies directed against the four enterotoxins A,B, C 1 and D to detect them under field conditions. Enterotoxins B and C 1 were detected in the same system by usig polyspecific monoclonal antibody to B and C 1. This indirect double sandwich ELISA was examined with success for detection of enterotoxins A, C 1 and D after their addition in different ,food samples and was also compared with competitive radioimmunoassay (RIA) using polyclonal serum directed against specific enterotoxin. Further, foods were artificially inoculated with toxigenic strains of S. aureus isolated from food samples and detection of enterotoxins was performed in these artificially contaminated foods by indirect double sandwich ELISA.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
