Abstract
A thermal shift assay (TSA) involves measuring the effect of a compound on the thermal stability of a protein as an indirect measure of ligand binding. In this chapter, we provide a protocol for a conventional TSA with recombinant/purified proteins using differential scanning fluorimetry (DSF), followed by a protocol for a Cellular Thermal Shift Assay (CETSA®), which measures the soluble cellular protein remaining after a transient heat shock of live cells to detect intracellular ligand binding.
Published Version
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