Indirect Competitive Immunoassay on a Blu-ray Disc for Digitized Quantitation of Food Toxins.

Abstract

We report herein a Blu-ray disc technology enabled immunoassay (namely, assay-on-a-Blu-ray) protocol for the quantitation of food toxins. In particular, commercial Blu-ray discs (BDs) are activated as substrates to create indirect competitive immunoassays with the aid of microfluidic channel plates for the quantitation of aflatoxins; an unmodified Blu-ray drive is employed to read the digitized signal (error counts generated from gold/silver-particle-enhanced binding sites); and a free disc-quality control software is adapted to process the raw data. The performance of this BD-based digital detection platform has been tested for the quantitation of aflatoxin B1 (AFB1) in spiked corn samples and validated with standard high-performance liquid chromatography measurements. The detection limit attained is as low as 0.27 ppb with a dynamic response range up to 200 ppb, which meets the standards established by government agencies worldwide for food products. We truly believe that the application potential of such a BD-technology-based, portable device for multiplex on-site quantitative analysis of food products as well as environmental and biomedical samples in real time is unlimited.