Indirect Competitive Enzyme-Linked Immunosorbent Assay for the Detection of Dibutyl Phthalate in White Wine, Compared With GC-MS

Abstract

Plasticizer has attracted more and more attention in China for the past 3 years, especially in Taiwan district. In this study, an indirect competitive enzyme-linked immunosorbent assay (icELISA) has been developed for the determination of a plasticizer dibutyl phthalate (DBP) in white wine. Dibutyl 4-aminophthalate coupled with OVA was synthesized as an immunogen to produce polyclonal antibodies against DBP. The antibody exhibited negligible cross-reactivity with other related compounds. The influence of several physicochemical parameters, such as coating procedure, organic solvent, competitive reaction time, and pH was investigated. The limit of detection was 64.5 ng/mL, which was sensitive enough for a screening assay. The linear range was 64.5–1,606.2 ng/mL with a correlation coefficient (R 2) of 0.996. The method was successfully applied to the determination of DBP in white wine. Recoveries were between 83.1 and 101.7 %. This immunoassay was highly specific, sensitive, rapid, simple, and suitable for DBP monitoring. The results obtained were compared with those obtained using gas chromatography–mass spectrometry (GC-MS), and a satisfied correlation coefficient of 0.928 was obtained by real sample detection.