INDICES OF MATRIX METALLOPROTEINASES (MMP-2, MMP-9, TIMP-1) WITH JUVENILE ARTHRITIS IN CHILDREN

Abstract

One of the perspectives of modern rheumatology is the study of matrix metalloproteinases (MMP) in juvenile arthritis--enzymes that play a key role in the process of joint destruction. To analyse the content of matrix MMP-2 and MMP-9 and their tissue inhibitor (TIMP-1) in blood serum and synovial fluid in various embodiments of juvenile arthritis in children. The study involved 82 children with juvenile arthritis, and 20 healthy children. The level of MMP-2, MMP-9 and TIMP-1 were determined in serum and synovial fluid by ELISA. It was found out that with any form of arthritis serum concentrations of MMP-2, MMP-9 and TIMP-1 was significantly higher than control values, but the level of MMP-2 in a subset of enthesitis-related arthritis, didn't differ from the control. Studied parameters in the synovial fluid were much higher than the serum level. With the development of uveitis TIMP-1 in blood serum was lower than in the absence of eye damage. On treatment of patients significant changes in the studied enzymes weren't established. On a good response to therapy the level of MMP-9 in serum decreased, on the lack of effectiveness - increased. The analysis results confirm the involvement of MMP-2, MMP-9 and TIMP-1 in the processes of inflammatory changes of the joints in juvenile idiopathic arthritis (JIA) and reactive arthritis, regardless the patients' sex or age.