Indicators of oxidative-nitrosative stress of men injured as a result of combat actions

Abstract

The article presents data on the state of lipid peroxidation and the activity of NO-synthase isoforms and arginase in lymphocytes and peripheral blood serum in men injured in combat (bullet and shrapnel wounds). 68 men injured as a result of hostilities (shrapnel and bullet wounds), who made up the main group, were examined. 48 practically healthy men were included in the control group. Patients of the main and control groups were divided into two age subgroups each: 20–39 years and 40–60 years. The MDA content in the blood serum of patients of both subgroups of the main group was 1.4 times higher than in the individuals of the corresponding subgroups of the control group (p<0.05). In peripheral blood lymphocytes, the MDA content in patients of both age subgroups of the main group was 1.3–1.4 times higher than in individuals of the corresponding subgroups of the control group (p<0.05; p<0.01). The activity of inducible (Ca2+-independent) de novo synthesis of NO significantly increases in blood serum and lymphocytes of men injured as a result of hostilities. At the same time, the activity of Ca2+-dependent NO-synthase in blood lymphocytes of men injured as a result of hostilities was 2.4 times lower compared to the values in practically healthy men (p<0.001), while it almost did not change in blood serum. It was confirmed that the inducible NOS can activate the processes of lipid peroxidation in men injured as a result of hostilities, as a direct significant correlation of high strength was recorded between the activity of Ca2+-independent iNOS and the MDA content (r=0.74; p<0.05). A significant correlation of medium strength was also established between arginase activity and MDA content (r=0.52; p<0.05). Keywords: malondialdehyde, NO-synthase, arginase.