Abstract

IN quantitative one-dimensional paper chromatography of amino-acids, the position of the various bands is generally determined either by running reference strips on the edges of the paper or by spraying lightly with weak ninhydrin solution. Neither method is entirely satisfactory. Reference strips are not always a reliable guide to cutting, particularly if the bands are close together, since the fronts may not be straight; the use of ninhydrin must give rise to some loss, which will fall most heavily on the acids present in least amount.

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