Indicator Displacement Assay-based Chemosensor Arrays for Saccharides using Off-the-shelf Materials toward Simultaneous On-site Detection on Paper

Yui Sasaki,Qi Zhou,Tsuyoshi Minami,Xiaojun Lyu

doi:10.1246/cl.200962

Abstract

Chemosensor arrays are useful analytical methods in supramolecular analytical chemistry, which discriminate molecular information such as species and their concentrations based on pattern recognition. Molecular self-assembled systems can provide not only multi-optical response patterns but also easy preparation of chemosensors. In this regard, dynamic covalent bonds play key roles in competitive assays (i.e., indicator displacement assay (IDA)). In this review, we propose an actual approach for simple IDA-based colorimetric and fluorescent chemosensor arrays utilizing only off-the-shelf materials. With chemometrics techniques, the IDA-based chemosensor arrays successfully recognized target saccharides. Toward on-site analysis, we further applied the IDA-based chemosensor array to a paper-based analytical device. The chemosensor array on the paper has achieved an accurate on-site analysis utilizing image processing without spectrophotometric instruments. The off-the-shelf material-based chemosensor arrays with pattern recognition techniques would be a novel analytical method for lab-free detections.

Highlights

Representative supramolecular sensors are defined as chemosensors, and the recognition information upon the detection of analytes can be shown through colorimetric or fluorescent responses.[1]
We propose an actual approach for simple indicator displacement assay (IDA)-based colorimetric and fluorescent chemosensor arrays utilizing only off-the-shelf materials
We devote ourselves to the qualitative and quantitative detection of saccharides by only using a small number of off-the-shelf materials.3436 As a further demonstration, the IDA-based chemosensor array has been applied to a paper-based analytical device toward on-site analysis.[37]

Summary

Introduction

Representative supramolecular sensors are defined as chemosensors, and the recognition information upon the detection of analytes can be shown through colorimetric or fluorescent responses.[1]. Saccharides are contained in foods and drinks and play essential roles in the human body, the detection of which is highly desired.[32,33] Focusing on the chemical structures of the saccharides, we realize that the high similarity of the saccharide structures could cause difficulty in accurate analysis To this end, we devote ourselves to the qualitative and quantitative detection of saccharides by only using a small number of off-the-shelf materials.3436 As a further demonstration, the IDA-based chemosensor array has been applied to a paper-based analytical device toward on-site analysis.[37] In this review, we describe the potential of the supramolecular chemosensors as a low-cost and user-friendly analytical method utilizing off-the-shelf materials and a paper substrate

Colorimetric Chemosensor Array

Fluorescence Chemosensor Array

Paper-based Chemosensor Array Device

Findings

Conclusions