Abstract
The Indian major carp, mrigal (Cirrhinus mrigala Ham.), sensitised with live Aeromonas hydrophila, were used to evaluate in vitro cellular immune-effector activities against A. hydrophila antigens. Fishes were maintained in the laboratory in four groups, namely, two control C1 (n = 10) and C2 (n = 10) and two test (treated) groups namely T1 (n = 10) and T2 (n = 10). Test fishes (T1 and T2) were inoculated (100 μl) intra-peritoneally (I/P) with live A. hydrophila at 2 × 107 cfu/ml for sensitisation. At 7th, 15th and 21st day post inoculation (DPI), fish (n = 2) from each tank were sampled, mononuclear cells from spleen were isolated and plated at 2 × 105 cells per well. The stimulation indices (SI) of leucocytes stimulated by crude somatic antigens (SAs) were observed to be much higher in the sensitised fish than in control of all DPI. However, stimulation was observed to be peak at 15 DPI. The in vitro percent cytotoxicity of sensitised leucocytes was observed to be 65.8% at 7 DPI, 38.1% at 15 DPI and 13.21% at 21 DPI. The results showed high specific effector activities by immune cells of Indian major carp mrigal in the initial 15 DPI. The study reveals for the first time the dynamic changes in specific immune-cellular activities of mrigal fish in initial infective phases, experimentally inoculated with live A. hydrophila.
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