Indexes of vitamin D deficiency in Japanese quail embryos.

Abstract

Effects of vitamin D deficiency (-D) on mineral homeostasis were investigated in Japanese quail embryos. The -D embryos from 1,25(OH)2D3-fed hens became progressively calcium deficient, as documented by hypocalcemia and reduced calcium accumulation by the skeleton, yolk sac, and allantoic fluid. Plasma phosphate was progressively elevated between days 11 and 15. Increased calcium accumulation by the skeleton, yolk sac, and allantoic fluid occurred between days 12 and 15 in +D embryos. Phosphate and adenosine 3',5'-cyclic monophosphate (cAMP) concentrations of allantoic fluid increased in +D embryos during the period of shell calcium mobilization. Further increases in phosphate and cAMP excretion into allantoic fluid occurred in -D embryos, although no calcium was absorbed from the shell. Renal 25(OH)D-1-hydroxylase activity increased between days 11 and 13, whereas the adenylate cyclase response to parathyroid hormone was lost in -D embryos by day 14. These changes in renal function are indicative of secondary hyperparathyroidism in the -D embryos. Differentiation of villus cavity and capillary covering cells occurred in the chorionic epithelium of -D embryos, but eggshell calcium was apparently not absorbed. In contrast, 75% of the total body calcium of newly hatched (+D) chicks was obtained from the eggshell. Thus the dissolution and/or transport of eggshell calcium is dependent on vitamin D in quail embryos.