Abstract
Incubation of liver macrophages with zymosan, phorbol ester and calcium ionophore A 23187 led to the formation of thromboxane, prostaglandin E 2 and prostaglandin D 2, whereas after external addition of arachidonic acid prostaglandin E 2 and prostaglandin D 2 only were found. This was confirmed by the use of labeled arachidonic acid given together with the stimuli. When the liver macrophages were prelabeled with [ 3H]arachidonic acid, and zymosan and [ 14C]arachidonic acid were added simultaneously, [ 3H]-label only was found in thromboxane whereas both [ 3H]- and [ 14C]-labeled PGE 2 and PGD 2 were detected in the cell medium. These data suggest that in cultured rat liver macrophages externally added arachidonic acid is accessible to the cyclooxygenase supplying prostaglandin H 2 for prostaglandin E 2 and D 2 synthesis but not for thromboxane synthesis.
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