Abstract
When using incremental gradient elution with a given column, the volume of the mixing vessel, the solvent period, the column dead volume and the flow-rate condition the resolution and analysis time for the separation of any given mixture. These factors affect the chromatographic performance due to the fact that they condition both the concentration profile of the solvent entering the column, the solute dispersion and the effective k′ values of the solute peaks as they are eluted. This paper describes results obtained from the investigation of the effect of these parameters and from the results suggests values for the variables that will afford a compromise between adequate resolution and acceptable analysis time. Examples of some separations using these chose conditions are included.
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