Abstract
Single stage conventional analysis of neutral protease activity present in certain tumour cell cytoplasmic fractions has been demonstrated to be totally misleading due to hidden errors introduced by the complex interactions of the protease with a cytoplasmic inhibitor of this enzyme. A novel system has been developed which enables the rapid simultaneous quantitative analysis of neutral protease activity and inhibitor activity. This technique employs an insoluble fluorescein-labelled substrate (polymeric collagen fibrils) and relies on the sensitive fluorimetric assay of solubilised fluorescein-labelled peptides. This technique has been termed “incremental analysis” and the advantages of incremental analysis over conventional analysis methods have been described in detail. The experimentally obtained results can readily be resolved graphically or by simple computation.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
