Increasing specificity of modern test-systems for diagnosis of ToRCH infections on example of IgM CMV

Abstract

The aim of the study was to Substantiate the format of tests for laboratory diagnosis of ToRCH-infections, to identify the causes of insufficient sensitivity and specificity of the ELISA tests on the example of determination of immunoglobulin M against cytomegalovirus, and to offer the solutions. Conclusions. The method of mucapture solves the problem of specificity, and the variant of sequential incubation with conjugates - the first incubation with Biotin, the second with streptavidin - enhances the signal by increasing the number of covalent bonds, which increases the sensitivity of the test.