Abstract
The side scattering light of a mammalian cell is caused, among other things, by the membranes of cell organelles. We suggest that cells with a high side scatter contain a large amount of mitochondria and a large endoplasmatic reticulum. In a simple approach we separate cells with a high side scatter via fluorescence activated cell sorting (FACS) to create sub populations with a higher productivity. We obtain cells with a high amount of mitochondria and a large endoplasmatic reticulum which could cause strong energy metabolism and high protein productivity. The advantage of this technique is that no staining dye or complex procedure is needed to reach the goal of increasing the productivity of a cell line.
Highlights
The side scattering light of a mammalian cell is caused, among other things, by the membranes of cell organelles
We suggest that cells with a high side scatter contain a large amount of mitochondria and a large endoplasmatic reticulum
The calculation of the specific productivity rates show that a higher specific productivity is achieved earlier in the sub populations than in the initial population (Fig. 1)
Summary
The side scattering light of a mammalian cell is caused, among other things, by the membranes of cell organelles. We suggest that cells with a high side scatter contain a large amount of mitochondria and a large endoplasmatic reticulum. In a simple approach we separate cells with a high side scatter via fluorescence activated cell sorting (FACS) to create sub populations with a higher productivity. We obtain cells with a high amount of mitochondria and a large endoplasmatic reticulum which could cause strong energy metabolism and high protein productivity. The advantage of this technique is that no staining dye or complex procedure is needed to reach the goal of increasing the productivity of a cell line
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