Abstract
In vitro assays for the β-N- acetylglucosaminyltransferases (GlcNAcTase) were performed on crude microsomal fractions prepared from 8-day chick embryo fibroblasts (8-day-CEF) and 16-day chick embryo fibroblasts (16-day-CEF) using [ 3H]mannose-labeled GlcNAc β1 → 2 Man α1 → 6 (GlcNAc β1 → 2 Man α1 → 3) Man β1 → 4 GlcNAc β1 → 4 (Fuc α1 → 6) GlcNAc-Asn and UDP-GlcNAc as substrates. 8-day-CEF synthesize preferentially triantennary complex type chains, whereas 16-day-CEF produce essentially tetraantennary complex type chains. Furthermore oligosaccharides containing the GlcNAc β1 → 4 Man α1 → 3 Man sequence represent 90% of the structures found in 16-day-CEF versus 30% in 8-day-CEF, indicating an increase in β-N- acetylglucosaminyltransferase IV activity during embryo development.
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