Abstract
The transport of 2-aminoisobutyric acid has been studied in stimulated rat lymphocytes. The capacity to accumulate 2-aminoisobutyric acid increased twofold after a 5 min incubation with phytohaemagglutinin (PHA) and concanavalin A (conA). It reached a maximum level which was 6–8 times the level of the control culture after 24 h of incubation with PHA. The early increased transport induced by conA could not be attributed to a decreased efflux, increased exchange diffusion or passive diffusion but reflected an increased rate of active influx. Kinetic analysis showed an increase in the maximum velocity of uptake ( V max) while no effect was observed on the apparent affinity ( Km). The increased uptake was dependent on protein synthesis and only partly dependent on the generation of metabolic energy. It is concluded that concanavalin A has no direct effect on the binding of 2-aminoisobutyric acid to the carrier and on the mobility or the number of carriers.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
