Abstract
L929 and MDCK cells acclimated to sorbitol, proline or taurine media can tolerate a NaCl load that they would be unable to stand when cultured in normal conditions. The protecting effect is observed only at high concentrations, between 100 and 300 mM. In that range, the effect is increasing with increasing concentration. The presence of the compound is not necessary, in the extracellular medium, to induce tolerance. These results are discussed in the framework of the theory considering preferential interactions of proteins with solvent components to explain the protecting effect of such compounds.
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