Abstract
Using the obese‐prone (OP) and obese‐resistant (OR) rat model, we studied the effects of diet induced obesity on peroxynitrite levels in various tissues/organs and expressions of lipolytic proteins in adipose tissue. After 8 weeks of high fat diet, animals were anesthetized and various tissues were harvested. Nitrotyrosine levels were analyzed by ELISA from the tissue homogenates. The protein levels of phosphorylated hormone‐sensitive lipase (phospho‐HSL) and perilipin, two key enzymes involve with lipolysis, in white adipose tissue were examined by Western blot analysis. Nitrotyrosine concentrations were 2–3 three times higher in all OP rat tissues we examined including skin, adipose tissue, skeletal muscle and small intestine. The protein level of phospho‐HSL in adipose tissue is as much as 20% lower in OP rats as compared to OR rats. As well, the protein levels of perliipin were also lower in OP rat adipose tissue as compared to OR rats. These results suggest a state of chronic systemic inflammation mediated by NOergic oxidant peroxynitrite may contribute to obesity development and attenuated lipolysis in OP rats. Supported by NIH (AT004620, and AT004504) and ADA 7‐07‐RA‐100 to S. Ma.
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