Abstract
Neodiosmin has poor aqueous solubility but exerts a good debittering effect and is a strong antioxidant with potential applications in foods, beverages, and pharmaceutical preparations. The water solubility of neodiosmin was greatly enhanced by forming a neodiosmin/β-cyclodextrin/lysine ternary inclusion complex. The inclusion type is described in detail in terms of its structural aspects using a phase diagram of solubility. The neodiosmin/β-cyclodextrin/lysine ternary inclusion complex was synthesized and characterized by thermal analysis and powder X-ray diffractometry (XRD). The molecular and fractal structures of the ternary complexes were investigated using 1H nuclear magnetic resonance spectroscopy (1H NMR) and Fourier transform infrared spectroscopy (FT-IR). Additionally, scanning electron microscopy (SEM) revealed that neodiosmin was embedded in a matrix of β-cyclodextrin with lysine. The results demonstrated that the inclusion complex was formed and that the solubility of neodiosmin was greatly improved. Additionally, the bitterness-masking power of this system was evaluated by a panel test using a series of limonin concentrations as a reference scale. The neodiosmin/β-cyclodextrin/lysine ternary inclusion complex showed the highest efficacy, and the bitterness attenuation was statistically significant.
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