Year-end Sale % OFF 
Abstract
Traumatic brain injury (TBI) has been reported to increase seizure susceptibility and also contribute to the development of epilepsy. However, the mechanistic basis of the development of increased seizure susceptibility and epilepsy is not clear. Though there is substantial work done using rats, data are lacking regarding the use of mice in the fluid percussion injury (FPI) model. It is unclear if mice, like rats, will experience increased seizure susceptibility following FPI. The availability of a mouse model of increased seizure susceptibility after FPI would provide a basis for the use of genetically modified mice to study mechanism(s) of the development of post-traumatic epilepsy. Therefore, this study was designed to test the hypothesis that, mice subjected to a FPI develop increased seizure susceptibility to a subconvulsive dose of the chemoconvulsant, pentylenetetrazole (PTZ). Three groups of mice were used: FPI, sham, and naïve controls. On day 30 after FPI, mice from the three groups were injected with PTZ. The results showed that FPI mice exhibited an increased severity, frequency, and duration of seizures in response to PTZ injection compared with the sham and naïve control groups. Histopathological assessment was used to characterize the injury at 1, 3, 7, and 30 days after FPI. The results show that mice subjected to the FPI had a pronounced lesion and glial response that was centered at the FPI focus and peaked at 3 days. By 30 days, only minimal evidence of a lesion is observed, although there is evidence of a chronic glial response. These data are the first to demonstrate an early increase in seizure susceptibility following FPI in mice. Therefore, future studies can incorporate transgenic mice into this model to further elucidate mechanisms of TBI-induced increases in seizure susceptibility.
Highlights
An estimated 1.7 million people in the U.S experience a traumatic brain injury (TBI) each year, 80,000 of which develop long-term disabilities and 50,000 of which are fatal (Faul, 2010)
The mechanistic basis of the development of increased seizure susceptibility and epilepsy is not clear.Though there is substantial work done using rats, data are lacking regarding the use of mice in the fluid percussion injury (FPI) model
The availability of a mouse model of increased seizure susceptibility after FPI would provide a basis for the use of genetically modified mice to study mechanism(s) of the development of post-traumatic epilepsy
Summary
An estimated 1.7 million people in the U.S experience a traumatic brain injury (TBI) each year, 80,000 of which develop long-term disabilities and 50,000 of which are fatal (Faul, 2010). TBI causes several neuropathological manifestations, including cognitive, emotional, physiological, and psychological deficits (Rosenthal et al, 1998; Junqué, 1999; Vakil, 2005; Nampiaparampil, 2008; Bales et al, 2009). To these deficits, another pathology often associated with TBI is increased seizure susceptibility and the development of epilepsy (D’Ambrosio and Perucca, 2004). Why TBI results in the development of epilepsy and increased seizure susceptibility remains largely unknown, several candidate mechanisms have been postulated, including: neurodegeneration, neuroplasticity, neuroinflammation, and connective tissue formation. The use of animal models that mimic these effects will aid in the understanding of the mechanisms of TBI and may provide help in the development of better treatment strategies
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
