Abstract
Background: 8-hydroxydeoxyguanosine (8-OHdG) is commonly used as a marker to evaluate oxidative DNA damage in disorders including chronic inflammatory diseases such as inflammatory periodontal pathologies. In the current study we hypothesized that the level of 8-OHdG in saliva increases by the periodontal destruction severity determined by clinical parameters as clinical attachment level (CAL).Materials and methods: A cross-sectional study was conducted on a sum of 60 age gender balanced; chronic periodontitis (CP) (n= 20), chronic gingivitis (CG) (n= 20) and healthy (H) (n= 20) individuals. Clinical periodontal parameters and salivary 8-OHdG levels were evaluated.Results: The mean 8-OHdG level in the saliva of the CP group was significantly higher than H and CG groups (p< 0.001). Statistically significant correlation was only observed between the salivary levels of 8-OHdG and age (p< 0.05), probing depth (PD) and CAL (p< 0.001) in CP group. However, when CP patients were classified according to their CAL levels (CAL≥ 3 mm (n= 11) and CAL<3 mm (n= 9)) statistically significant correlation was only observed between the salivary levels of 8-OHdG and CAL≥ 3 mm patients (p< 0.001).Conclusion: We suggest that elevated salivary levels of 8-OHdG may be a marker for disease activity and it may reflect indirectly disease severity parameters such as CAL.
Highlights
Periodontitis is defined as an inflammation of the periodontium that extends beyond the gingival tissue and produces destruction of the connective tissue attachment of the teeth [1,2]
CP1 group consisted of chronic periodontitis patients with clinical attachment level (CAL 3 mm; moderate+severe) (n:11) where CP2 (n:9) group consisted of chronic periodontitis patients with CAL< 3 mm
plaque index (PI), gingival index (GI) scores of the Chronic periodontitis group (CP) and Chronic gingivitis group (CG) groups were significantly higher than the control group (p < 0.001)
Summary
Periodontitis is defined as an inflammation of the periodontium that extends beyond the gingival tissue and produces destruction of the connective tissue attachment of the teeth [1,2]. Tissue damage in inflammatory periodontal pathologies can be mediated by ROS resulting from the physiological activity of polymorphonuclear leukocytes during phagocytosis of periodontopathic bacteria, and can occur through a number of mechanisms such as protein disruption [7], lipid peroxidation [8,9], induction of proinflammatory cytokines [6] and DNA damage [10]. Previous studies [20,21,22,23,24,25] indicated a possible relationship between salivary levels of 8-OHdG and diseased periodontium. We investigated the salivary 8-OHdG levels in periodontitis patients, gingivitis patients, and healthy controls and explored correlations between these oxidative DNA damage markers and clinical parameters of periodontitis and age
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