Abstract
Abstract Rice bean (Vigna umbellata L.) sprouts are richer in metabolites and biological activities after germination. The total polyphenol and total flavonoid contents were analyzed during the sprouting process. The antioxidant activities of the sprouts were tested using 1,1-diphenyl-2- picrylhydrazyl radical (DPPH) screening assay. To determine the reason for increased activity, quantitative analysis of 8 flavonoids, in the germination process of methyl jasmonate (MeJA)-treated and control groups, was performed using high-performance liquid chromatography coupled with diode array detection (HPLC/DAD) and partial least squares discriminant analysis (PLS/DA). The metabolites in MeJA-treated beans shows that addition of 100.0 μmol MeJA significantly increased the total flavonoid and polyphenol contents during the sprouting process. DPPH screening assay and analytical results show that MeJA has a significant influence on the production of secondary metabolites in sprouts between the 4th and 6th days, thereby inducing increased antioxidant activity. Flavonoids rutin, kaempferol-3-O-rutinoside, daidzin, and genistin are further identified as the main contributors to the increased DPPH screening activity.
Highlights
Rice bean (Vigna umbellata L.), known as the climbing mountain bean, mambi bean, and oriental bean, is native to Southeast Asia
No obvious differences were observed in the physical appearances of methyl jasmonate (MeJA)-treated and control group sprouts, MeJA treatment significantly affected polyphenol metabolism
Polyphenols and antioxidant activity of rice bean sprouts induced by Methyl Jasmonate in the sprouts
Summary
Rice bean (Vigna umbellata L.), known as the climbing mountain bean, mambi bean, and oriental bean, is native to Southeast Asia. It is consumed as a vegetable or as a pulse mixed with rice (Saikia et al, 1999). Phenolic acids and flavonoids are major classes of metabolites found in rice bean. Three phenolic acids (p-coumaric acid, ferulic acid, and sinapic acid) and five flavonoids (catechin, epicatechin, vitexin, isovitexin, and quercetin) have been identified in different rice bean samples. The effects of MeJA on the production of secondary metabolites (alkaloids, polysaccharides, and flavonoids) have been reported (Jiao et al, 2018). The results wish to be beneficial to utilize rice bean sprouts as the raw materials for functional foods, health products, and cosmetics
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