Abstract
SummaryThe absorption of phenolsulfon-phthalein from canine Heidenhain pouches before and after the topical application of 4M urea was determined. In addition, associated changes in the net fluxes of H2O, H+, K+ and Cl− and the net and bidirectional fluxes of Na+ were assayed to document disruption of the mucosal barrier. PSP absorption before urea instillation averaged 0.13 ± 0.4 μM/30 min/100 cm2 mucosal urea. Following urea, an average of 0.63 ± 0.06 μM/30 min/100 cm2 mucosal area was absorbed. Associated ionic and water flux rates demonstrated significantly increased “back diffusion” of H+ and influx of Na+, K+ and water into the lumen. The observation of over a fourfold increase in PSP absorption after urea instillation indicates that mucosal permeability is increased and suggests that increased effective pore area is responsible. It is concluded that PSP should not be used as a volume marker in studies in which the integrity of the gastric mucosal barrier is altered.
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Schedule a callMore From: Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)
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