Abstract

The hypertrophic adenoid may promote chronic suppurative otitis media in children as it fulfills its immune function. The number of lymphocytes in the adenoid and their cooperation in the immune response depend of on their proliferation and migration to the effector sites. Interleukin 7 (IL-7) is essential for the normal development and function lymphocytes. IL-7 plays pivotal role for activation and proliferation of T and B cells. The heterodimeric interleukin-7 receptor (IL-7R) is composed of the IL-7Rα (127) and the common cytokine receptor γc (CD132). The aim of this study was to evaluate the percentage of lymphocytes T (CD4+ and CD8+) with IL-7R (CD127 and CD132) expression in hypertrophic adenoid in children suffering with otitis media with effusion for a duration of 3 months. Adenoid excised due to hypertrophy with or without chronic otitis media with effusion was used as study material. CD4+ CD127+, CD4+132+, CD8+CD127+ and CD8+CD132+ cell subpopulations were identified using monoclonal antibodies and flow cytometry. The percentage of CD4+ and CD8+ T cells with CD127 receptor expression in hypertrophic adenoid of children with otitis media with effusion was statistically significantly higher than in hypertrophic adenoid group. The percentage of CD4+ T cells with CD132 expression in the study group was statistically significantly higher than in the reference group. The percentage of CD8+ T cells with CD132+ expression was not statistically different in both groups. The increased percentage of T lymphocytes with IL-7R expression (CD127 and CD132) in hypertrophic adenoid seems to influence the quantity of lymphocytes and upset the immunological function of tonsils which can influence the course of otitis media with effusion.

Highlights

  • Interleukin 7 (IL-7) plays a basic role in the development of lymphocytes in the thymus, in the production of memory T cells and in the homeostasis of peripheral T cells [1,2,3]

  • The increased percentage of T lymphocytes with interleukin-7 receptor (IL-7R) expression (CD127 and CD132) in hypertrophic adenoid seems to inXuence the quantity of lymphocytes and upset the immunological function of tonsils which can inXuence the course of otitis media with eVusion

  • The study material contained adenoid excised from 35 children (18 girls and 17 boys, aged from 3 to 17) suVering from adenoid hypertrophy coexisting with otitis media with eVusion (OME) lasting at least 3 months

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Summary

Introduction

Interleukin 7 (IL-7) plays a basic role in the development of lymphocytes in the thymus, in the production of memory T cells and in the homeostasis of peripheral T cells [1,2,3]. The expression of IL-7R (CD127 and CD132) can be found in naive CD4+/CD8+ T cells and CD4+/CD8+ memory T cells [7,8,9,10]. The signal transmitted through the IL-7R induces increased expression of apoptotic proteins, mainly Bcl-2 [10, 12,13,14], cell proliferation [13, 15], antigen-independent naive T-cell proliferation, strong expansion of memory T cells (eVector) in the presence and lack of antigen [7, 8, 16]

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