Increased nucleoside diphosphate kinase activity induces white spot syndrome virus infection in Litopenaeus vannamei.

Peng-Fei Liu,Yin Wu,Qing-Hui Liu,Jie Huang,Gao-Feng Qiu

doi:10.1371/journal.pone.0175741

Abstract

Nucleoside diphosphate kinase (NDK), which has the same sequence as oncoprotein (OP) in humans, can induce nucleoside triphosphates in DNA replication by maintenance of the deoxynucleotide triphosphate (dNTP’s) and is known to be regulated by viral infection in the shrimp Litopenaeus vannamei. This paper describes the relationship between NDK and white spot syndrome virus (WSSV) infection. The recombinant NDK was produced by a prokaryotic expression system. WSSV copy numbers and mRNA levels of IE1 and VP28 were significantly increased in shrimp injected with recombinant NDK at 72 h after WSSV infection. After synthesizing dsRNA-NDK and confirming the efficacy of NDK silencing, we recorded the cumulative mortality of WSSV-infected shrimp injected with NDK and dsRNA-NDK. A comparison between the results demonstrated that silencing NDK delayed the death of shrimps. These findings indicate that NDK has an important role influencing the replication of WSSV replication in shrimp. Furthermore, NDK may have potential target as a new therapeutic strategy against WSSV infection in shrimp.

Highlights

Nucleoside diphosphate kinase (NDK) is an essential enzyme which is required for the production of nucleoside triphosphates by a reaction that catalyses the transfer of a phosphoryl group from nucleoside 50-triphosphates to nucleoside 50-diphosphates, it plays a key role in maintaining intracellular energy resources [1, 2]
On analyzing the amino acid sequence, we discovered that it was similar to the human oncoprotein nm23, and when shrimps were infected by white spot syndrome virus (WSSV), NDK
Recombinant NDK was successfully expressed in E. coli

Summary

Introduction

Nucleoside diphosphate kinase (NDK) is an essential enzyme which is required for the production of nucleoside triphosphates by a reaction that catalyses the transfer of a phosphoryl group from nucleoside 50-triphosphates to nucleoside 50-diphosphates, it plays a key role in maintaining intracellular energy resources [1, 2]. Members of the Nme gene family encode nucleoside diphosphate kinases [3]. Recent research indicates that members of the nm family display multiple functions in diverse biological processes such as signal transduction, growth control, differentiation, cell migration, and the promotion of cancer [4,5,6]. The sequence of the shrimp NDK was highly conserved with 40 and 60% identity among eukaryotic and bacterial NDKs, respectively. On analyzing the amino acid sequence, we discovered that it was similar to the human oncoprotein nm, and when shrimps were infected by white spot syndrome virus (WSSV), NDK

Objectives

Methods

Results

Discussion

Conclusion