Increased nuclear expression of nuclear factor kappa-B p65 subunit in the eutopic endometrium and ovarian endometrioma of women with advanced stage endometriosis

Abstract

ObjectiveTo evaluate whether the nuclear expression of nuclear factor kappa-B (NF-κB) p65 subunit is increased in the eutopic endometrium and/or the ovarian endometrioma of women with advanced stage endometriosis.DesignCase-controlled laboratory study in a university hospital.Materials and MethodsWe recruited a total of 66 women who underwent hysterectomy due to carcinoma in situ (CIS) of the uterine cervix (n=32) or advanced stage endometriosis (n=34) as well as 30 nulliparous women who underwent ovarian cystectomy due to endometrioma. Immunohistochemistry was performed to compare the nuclear NF-κB p65 subunit immunoreactivity between women with and without advanced stage endometriosis. The nuclear NF-κB p65 subunit expression and DNA binding were analyzed utilizing Western blotting, enzyme-linked immunosorbent assay (ELISA), and electrophoretic mobility shift assay (EMSA) in endometrial cells treated with tumor necrosis factor-alpha (TNF-α) or interleukin-1beta (IL-1β).ResultsThe immunoreactivity of the nuclear NF-κB p65 subunit was significantly increased in the eutopic endometrium as well as in the ovarian endometrioma of women with advanced stage endometriosis compared to the CIS group. In vitro treatment of endometrial cells with TNF-α and IL-1β led to a significant increase of nuclear NF-κB p65 subunit expression and DNA binding.ConclusionThe nuclear expression of NF-κB p65 is increased in the eutopic endometrium and ovarian endometrioma of women with advanced stage endometriosis, which strongly suggest that NF-κB signaling plays a crucial role in the pathogenesis and/or pathophysiology of endometriosis. ObjectiveTo evaluate whether the nuclear expression of nuclear factor kappa-B (NF-κB) p65 subunit is increased in the eutopic endometrium and/or the ovarian endometrioma of women with advanced stage endometriosis. To evaluate whether the nuclear expression of nuclear factor kappa-B (NF-κB) p65 subunit is increased in the eutopic endometrium and/or the ovarian endometrioma of women with advanced stage endometriosis. DesignCase-controlled laboratory study in a university hospital. Case-controlled laboratory study in a university hospital. Materials and MethodsWe recruited a total of 66 women who underwent hysterectomy due to carcinoma in situ (CIS) of the uterine cervix (n=32) or advanced stage endometriosis (n=34) as well as 30 nulliparous women who underwent ovarian cystectomy due to endometrioma. Immunohistochemistry was performed to compare the nuclear NF-κB p65 subunit immunoreactivity between women with and without advanced stage endometriosis. The nuclear NF-κB p65 subunit expression and DNA binding were analyzed utilizing Western blotting, enzyme-linked immunosorbent assay (ELISA), and electrophoretic mobility shift assay (EMSA) in endometrial cells treated with tumor necrosis factor-alpha (TNF-α) or interleukin-1beta (IL-1β). We recruited a total of 66 women who underwent hysterectomy due to carcinoma in situ (CIS) of the uterine cervix (n=32) or advanced stage endometriosis (n=34) as well as 30 nulliparous women who underwent ovarian cystectomy due to endometrioma. Immunohistochemistry was performed to compare the nuclear NF-κB p65 subunit immunoreactivity between women with and without advanced stage endometriosis. The nuclear NF-κB p65 subunit expression and DNA binding were analyzed utilizing Western blotting, enzyme-linked immunosorbent assay (ELISA), and electrophoretic mobility shift assay (EMSA) in endometrial cells treated with tumor necrosis factor-alpha (TNF-α) or interleukin-1beta (IL-1β). ResultsThe immunoreactivity of the nuclear NF-κB p65 subunit was significantly increased in the eutopic endometrium as well as in the ovarian endometrioma of women with advanced stage endometriosis compared to the CIS group. In vitro treatment of endometrial cells with TNF-α and IL-1β led to a significant increase of nuclear NF-κB p65 subunit expression and DNA binding. The immunoreactivity of the nuclear NF-κB p65 subunit was significantly increased in the eutopic endometrium as well as in the ovarian endometrioma of women with advanced stage endometriosis compared to the CIS group. In vitro treatment of endometrial cells with TNF-α and IL-1β led to a significant increase of nuclear NF-κB p65 subunit expression and DNA binding. ConclusionThe nuclear expression of NF-κB p65 is increased in the eutopic endometrium and ovarian endometrioma of women with advanced stage endometriosis, which strongly suggest that NF-κB signaling plays a crucial role in the pathogenesis and/or pathophysiology of endometriosis. The nuclear expression of NF-κB p65 is increased in the eutopic endometrium and ovarian endometrioma of women with advanced stage endometriosis, which strongly suggest that NF-κB signaling plays a crucial role in the pathogenesis and/or pathophysiology of endometriosis.