Abstract
The plastid rbcL gene, encoding the large subunit of ribulose-1, 5-bisphosphatecarboxylase, in higher plants is transcribed from a σ70 promoter by the eubacterial-type plastid-encoded plastid RNA polymerase (PEP). In vitro studies have confirmed the importance of the -35 and -10 box spacing and sequence for the rbcL promoter strength (1, 2). Furthermore, sequences positioned between nucleotides -16 and -102 relative to the rbcL transcription initiation site were proposed to function in maize as a binding site for the chloroplast DNA-binding factor 1 (CDF1) sequence-specific transcription factor (3). To identify regulatory elements outside the rbcL -10/-35 promoter core, transplastomic tobacco plants were constructed with uidA reporter genes expressed from rbcL promoter derivatives.
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