Abstract

Chloride ion-selective microelectrodes were used to measure the intracellular chloride activity of Lymnaea stagnalis neurons. Values ranged from 3 to 23.5 m M in different cells. Neurons with inhibitory postsynaptic potentials had significantly lower chloride activity levels than cells without such inputs. Bicarbonate-buffered saline reduced intracellular chloride levels compared with those in Hepes-buffered saline. Frescon (Shell Chemicals) produced an increase in intracellular chloride activity in 14 out of 22 neurons (mean rate of increase, 0.09 m M/min). This increase was insensitive to changes in the membrane potential: the rate of increase was reduced in bicarbonate-buffered saline. The mechanism of Frescon action is discussed in terms of a bicarbonate/chloride exchange mechanism.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.