Increased Inter-Colony Fusion Rates Are Associated with Reduced COI Haplotype Diversity in an Invasive Colonial Ascidian Didemnum vexillum

Kirsty F Smith,S Craig Cary,Yasunori Saito,Chrissen E C Gemmill,Lauren Stefaniak,Andrew E Fidler,Sharyn Jane Goldstien

doi:10.1371/journal.pone.0030473

Abstract

Considerable progress in our understanding of the population genetic changes associated with biological invasions has been made over the past decade. Using selectively neutral loci, it has been established that reductions in genetic diversity, reflecting founder effects, have occurred during the establishment of some invasive populations. However, some colonial organisms may actually gain an ecological advantage from reduced genetic diversity because of the associated reduction in inter-colony conflict. Here we report population genetic analyses, along with colony fusion experiments, for a highly invasive colonial ascidian, Didemnum vexillum. Analyses based on mitochondrial cytochrome oxidase I (COI) partial coding sequences revealed two distinct D. vexillum clades. One COI clade appears to be restricted to the probable native region (i.e., north-west Pacific Ocean), while the other clade is present in widely dispersed temperate coastal waters around the world. This clade structure was supported by 18S ribosomal DNA (rDNA) sequence data, which revealed a one base-pair difference between the two clades. Recently established populations of D. vexillum in New Zealand displayed greatly reduced COI genetic diversity when compared with D. vexillum in Japan. In association with this reduction in genetic diversity was a significantly higher inter-colony fusion rate between randomly paired New Zealand D. vexillum colonies (80%, standard deviation ±18%) when compared with colonies found in Japan (27%, standard deviation ±15%). The results of this study add to growing evidence that for colonial organisms reductions in population level genetic diversity may alter colony interaction dynamics and enhance the invasive potential of newly colonizing species.

Highlights

Human mediated transfer of species into regions beyond their native range, and the resulting ecosystem perturbations, are major contributors to currently accelerating rates of indigenous biodiversity loss and species extinction [1,2,3]
The New Zealand D. vexillum populations sampled contained as few as three cytochrome oxidase I (COI) haplotypes
These data suggest an interpretation that D. vexillum experienced a significant reduction in genetic diversity when colonizing New Zealand, presumably due to a founder effect

Summary

Introduction

Human mediated transfer of species into regions beyond their native range, and the resulting ecosystem perturbations, are major contributors to currently accelerating rates of indigenous biodiversity loss and species extinction [1,2,3]. Population genetic theory predicts that the small founder populations typical of invasive species, at least in the initial colonization stages, contain an unrepresentative portion of the total genetic variation present in the source population [7,8] and that further stochastic losses of allelic diversity (i.e. genetic drift) are anticipated in such small populations [4,7,9] Such non-adaptive changes in the genetics of newly colonizing species are expected to have both genotypic and phenotypic consequences, possibly including deleterious inbreeding depression [10,11]. In some cases invasive populations appear to have increased genetic diversity in their new environment which has been attributed to admixture of lineages from multiple native populations with differing genetic profiles [16,17,18]

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Conclusion