Abstract
Cryopreservation and thawing of embryos has undergone little change over time. Current conventional protocols for blastocysts utilize 2 step dehydration in glycerol and sucrose followed by slow cooling to -35–40C and plunging into liquid nitrogen. The most common thawing protocol is a 2–3 step removal of glycerol and sucrose. Some protocols suggest that the equilibration and removal of cryoprotectant, should be performed at 370C. This report is a retrospective analysis of the data from 2 non-contemporaneous phases of a blastocyst freezing-thawing program utilizing a 2-step dehydration method and either a 2 step re-hydration or a slow, 7 step re-hydration, both performed at room temperature.
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