Abstract
Cyclophosphamide (CY) treatment of F 1 hybrid mice increases their susceptibility to attack by parental-strain lymphoid cells. The donor cells may contribute to this increased susceptibility either by a more vigorous response to the host antigens, or by an increase in their colonization of the host's tissues. We have assessed the responsiveness of the donor cells in the CY-treated host through the use of a local graft-versus-host (GVH) assay. This assay is not influenced by changes in the capacity of donor cells to colonize host tissues, and thus colonization has been eliminated as a variable. In this assay donor lymph node tissue is grafted onto the cut surface of host kidney, and a local GVH reaction is indicated by enlargement of the donor tissue. We show that treatment of F 1 hosts with CY (100 mg/kg) 24 hr prior to grafting leads to increased responsiveness of the donor cells as measured by enlargement of the donor tissue.
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