Abstract
ABSTRACT Media prepared from agricultural by-products including malt, wheat bran, corn steep liquor and soybean flour, were found to be an excellent substrate for proliferation of Aspergillus niger #57. Glucoamylase yields obtained using various combinations of the above materials were ten to fifteen times higher than those obtained uisng semi-synthetic media. A medium comprising 10%wheat bran extract and 8% defatted soybean flour gave the highest enzyme yield of 42 IU/ml as compared with a yield of 2.8 IU/ml obtained using optimal concentrations of glucose and peptone.
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