Abstract
Auxotrophic mutants of Ustilago hordei were recovered after UV irradiation of a wild-type culture, inositol starvation of an inositol-requiring culture and a combination of the two methods. 1.9% of the survivors of UV irradiation followed by inositol starvation were found to carry a requirement additional to inositol. Inositol starvation by itself gave only 0.27% recovery while UV irradiation alone was even less efficient.
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