Abstract
We measured pools of soluble and sedimentable actin in hamster islets using a new DNase I binding/immunoprecipitation assay. Islets from fed and fasted hamsters contained the same amount of actin when expressed per microgram of protein. In three experiments the sedimentable (filamentous) actin increased significantly in islets from fasted hamsters. Recovery determinations demonstrate that during fasting the sedimentable actin pool (F) is increased as the soluble (G) actin pool decreases. These results suggest that the microfilament system is affected by the metabolic state of hamsters and may be responsible, in part, for inhibiting insulin secretion during fasting.
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