Increased expression of the intercellular adhesion molecule-1 (ICAM-1) on peripheral blood neutrophils in acute pancreatitis

Abstract

PurposeConsidering the important role of neutrophils’ activation in the pathogenesis of acute pancreatitis (AP), the aim of our study was to evaluate the expression of leukocytes’ adhesion molecules in patients with AP. Patients/methodsThirty-five patients (16 women and 19 men; age 32–77 years, median 56 years) with AP were prospectively included into our study. The absolute number of leukocytes was estimated by haematologic analyser. Surface neutrophils antigens (CD) were assayed by the direct fluorescence method for whole blood, using a flow cytometer. ResultsAt the day 1, significant increase of ICAM-1 expression was found in patients with severe AP (S-AP) (7280mm−3 vs 2850mm−3 in healthy control; p<0.05). In the days 2, 3 and 5 it sharply decreased and peaked again to 4860mm−3 at the day 10. In patients with mild AP (M-AP), not significant elevation of ICAM-1 quickly returned to normal level. In both forms of AP, neutrophil CD62L (L-selectin) expression reached the highest level at the day 1 (8800mm−3 and 9020mm−3, respectively in M-AP and S-AP, in comparison to 3400mm−3 in control; p<0.05). Expression of CD69 (neutrophils’ marker of early activation) significantly increased in both M-AP and S-AP. ConclusionsWe have found an early and significant increase of peripheral blood neutrophil CD54/ICAM-1 expression, specific for S-AP but not for M-AP. It may provide a good marker predicting severe course of pancreatitis.