Abstract
BackgroundMicroRNA-222 (miR-222) has been shown to play a potential oncogenic role in bladder cancer. The aim of this study was to evaluate the expression of miR-222 in bladder cancer and its potential relevance to clinicopathological characteristics and patient survival.MethodsSurgical specimens of cancer tissue and adjacent normal tissue were obtained from 97 patients with bladder cancer. The relative expression levels of miR-222 in the cancer and the normal adjacent tissue were measured by quantitative reverse-transcriptase PCR. We analyzed their correlation with clinicopathological parameters and prognostic value.ResultsThe expression level of miR-222 was significantly higher in tumor tissues than in corresponding non-cancerous tissues (5.46 ± 1.45 versus 1.92 ± 0.65, P < 0.0001), and a high expression of miR-222 was found to be significantly associated with tumor grade (P = 0.003) and tumor stage (P = 0.005). The miR-222 expression level was classified as high or low in relation to the median value (cutoff value = 5.15). Kaplan-Meier analysis showed that patients with higher levels of miR-222 had significantly poorer survival than those with lower expression of this miRNA in patients, with a 5-year overall survival of 29.53% and 52.75%, respectively (P = 0.0034). In the multivariate Cox proportional hazards analysis, which included miR-222 level, tumor grade, tumor stage, and tumor number, high miR-222 expression was independently associated with poor survival (P < 0.001; hazard ratio 6.17; 95% CI 2.33 to 10.39).ConclusionmiR-222 overexpression is involved in the poor prognosis of bladder cancer and can be used as a biomarker for selection of cases requiring special attention.
Highlights
MicroRNA-222 has been shown to play a potential oncogenic role in bladder cancer
Expression of miR-222 in bladder cancer tissues by quantitative reverse-transciptase polymerase chain reaction (qRT-PCR) We examined miR-222 protein expression in 97 pairs of bladder cancer tissues and the corresponding nonobserved between miR-222 expression and other clinicopathologic variables such as age, gender, tumor size, and number of tumors
Increased miR-222 expression in bladder cancer was found to be significantly associated with tumor grade (Ta-T1 versus ≥ T2: 4.19 ± 1.32 versus 8.11 ± 1.46, P = 0.003) and tumor stage (G1/2 versus G3: 3.42 ± 1.27 versus 7.91 ± 1.57, P = 0.005)
Summary
MicroRNA-222 (miR-222) has been shown to play a potential oncogenic role in bladder cancer. The aim of this study was to evaluate the expression of miR-222 in bladder cancer and its potential relevance to clinicopathological characteristics and patient survival. The incidence of bladder cancer increases with age, peaking between 50 and 70 years, and the disease is approximately three times more common in males than in females [2]. The expression of miR-222 in bladder cancer and its prognostic values still remain unclear. The aim of the present study is to evaluate the clinical significance of miR-222. The expression level of the miR-222 was measured in both adjacent normal tissue and cancerous tissue. The correlation between the expression level of miR-222 and clinicopathological characters was analyzed. The influence of miR-222 on the prognosis of bladder cancer patients was estimated
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