Increased expression of human leukocyte antigen-DR on pulmonary macrophages in bronchopulmonary dysplasia.

Abstract

We used an immunoperoxidase method to examine the expression of the immune activation marker HLA-DR on pulmonary tissue obtained at autopsy from 14 patients dying of bronchopulmonary dysplasia. Controls consisted of 16 age-matched, sex-matched children dying of noncardiac, nonrespiratory, noninfectious illnesses or as a result of motor vehicle accidents. We did not observe aberrant expression of HLA-DR on pulmonary endothelial cells. Positive staining appeared exclusively on macrophages. We quantitated the expression of antigen by counting the number of positive macrophages and total macrophages/monocytes per high power field. Bronchopulmonary dysplasia patients displayed significantly greater numbers of both positive and total macrophages compared with the control group (p < 0.05). The percent positive macrophages also was significantly higher in the bronchopulmonary dysplasia patients (p < 0.005). We also examined a group of patients dying with infant respiratory distress syndrome. There was no significant difference in number of total macrophages in this group compared with age-matched controls.