Increased EHHADH Expression Predicting Poor Survival of Osteosarcoma by Integrating Weighted Gene Coexpression Network Analysis and Experimental Validation.

Juncheng Cui,Jinxin Li,Dongyang Qian,Guoliang Yi,Yangtao Li

doi:10.1155/2021/9917060

Abstract

Enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase (EHHADH), a member of the 3-hydroxyacyl-CoA dehydrogenase family, were previously demonstrated to be involved in the tumorigenesis of various cancer types. This study is aimed at determining of the diagnostic and prognostic value of EHHADH in osteosarcoma (OS). The overexpression of EHHADH was found both in OS and also other sarcoma types, and according to the retrospective cohort study, the EHHADH level was related to the overall survival and disease-free survival of the OS patients. Furthermore, knockdown of EHHADH under the influence of EHHADH small interfering RNA significantly suppressed the proliferation ability of the tumor cells. Moreover, EHHADH overexpressed was found in human OS tissues. In summary, the progression of OS could be enhanced by EHHADH, which may be a potential diagnostic and prognostic biomarker for OS patients.

Highlights

Osteosarcoma (OS) is one of the commonly occurring malignant tumors in bone tissues [1]
OS is derived from the mesenchymal cell line, and the frequent growth of the tumor is associated with the development of tumor osteoid and bone tissue through the cartilage stage [2]
Various studies have reported that several tumor-related diseases are enriched with Enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase (EHHADH), which is of great importance for the progression of cancers [9, 10]

Summary

Introduction

Osteosarcoma (OS) is one of the commonly occurring malignant tumors in bone tissues [1]. Various studies have reported that several tumor-related diseases are enriched with EHHADH, which is of great importance for the progression of cancers [9, 10]. It is reasonably assumed that EHHADH is involved in the development of tumor-related diseases. According to the retrospective cohort study, the EHHADH level was related to the disease-free survival and BioMed Research International. Term hsa04966:Collecting duct acid secretion hsa04964:Proximal tubule bicarbonate reclamation hsa04146:Peroxisome hsa03320:PPAR signaling pathway hsa01212:Fatty acid metabolism hsa01200:Carbon metabolism hsa01130:Biosynthesis of antibiotics hsa01100: Metabolic pathways hsa00650:Butanoate metabolism hsa00640:Propanoate metabolism hsa00630:Glyoxylate and dicarboxylate metabolism hsa00620:Pyruvate metabolism hsa00410:beta-Alanine metabolism hsa00380:Tryptophan metabolism hsa00330:Arginine and proline metabolism hsa00280:Valine, leucine and isoleucine degradation hsa00260:Glycine, serine and threonine metabolism hsa00250: Alanine, aspartate and glutamate metabolism hsa00071:Fatty acid degradation hsa00020:Citrate cycle (TCA cycle). The clinical importance of EHHADH level in human OS and the regulatory effect of EHHADH on OS cell proliferation were explored

Materials and Methods

Results and Discussion

Conclusions