Abstract
Wound healing in muscle involves the deposition of collagen, but it is not known whether this is achieved by changes in the synthesis or the degradation of collagen. We have used a reliable flooding dose method to measure collagen synthesis rate in vivo in rat abdominal muscle following a surgical incision. Collagen synthesis rate was increased by 480% and 860% on days 2 and 7 respectively after surgery in the wounded muscle compared with an undamaged area of the same muscle. Collagen content was increased by approximately 100% at both day 2 and day 7. These results demonstrate that collagen deposition during wound healing in muscle is achieved entirely by an increase in the rate of collagen synthesis.
Highlights
Wound healing involves a series of processes which have traditionally been divided into four phases, haemostasis, inflammation, proliferation and remodelling [1,2]
Its validity depends crucially on all free proline pools in the body being flooded by the large dose of proline that was injected, so that the specific activity of free proline rapidly reaches a steady state value which is similar in all tissues and in the plasma and is maintained relatively constant throughout the 30 minute period of incorporation of labelled proline into protein
This is analogous to the flooding dose phenylalanine method [14] that is commonly used to measure the average synthesis rate of mixed proteins in tissues and was used in our previous experiments to measure muscle protein synthesis rate during wound healing [3,4]
Summary
Wound healing involves a series of processes which have traditionally been divided into four phases, haemostasis, inflammation, proliferation and remodelling [1,2]. We have previously measured the rate of protein synthesis in muscle during the healing of a surgical wound in vivo and found a substantial increase (70 – 150%), starting 48 hours after the operation and continuing at least until day 7 [3,4]. This increase in protein synthesis was not affected by malnutrition [4], suggesting that increased protein synthesis has a high biological priority. It is not known which proteins are involved in this accelerated protein synthesis, nor whether all protein fractions are affected
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